BIOL102 Outline Final Applied Lab Project and final project - April 2018

Asked by sharpie
Dated: 10th Jul'18 05:57 AM
Bounty offered: $45.00

Outline Final Applied Lab Project

Instructions

Include the following in your outline:

Name of enzyme you will use

Name of organism (if applicable)

The substrate and products in the chemical reaction

Method for measuring enzyme activity

Treatment: acidic fluid(s), pH, length of exposure, how you will treat your samples

The control(s) in the experiment

Hypothesis

How you will present your data (table and/or type of graph)

Anything else you would like to get feedback on before you start your experiment

Final Applied Lab Project

Instructions

Final Applied Lab Project (1 credit Lab Component)

Addresses course outcomes 1-4:

recognize and explain how the scientific method is used to solve problems

make observations and discriminate between scientific and pseudoscientific explanations

weigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific method

use knowledge of biological principles, the scientific method, and appropriate technologies to ask relevant questions, develop hypotheses, design and conduct experiments, interpret results, and draw conclusions

This is the culminating lab assessment in BIOL 102. It is designed to assess your ability to apply the principles of the scientific method.

The Effect of low pH on Enzyme Activity

Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, information about pH and enzymes in the text book and course modules, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), or you can search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references).

As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the text book, modules, lab manual and potentially additional information sources.

Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for example, a low pH value could change the shape of the active site on the enzyme protein), without directly participating in the reaction.

Lab Materials

You may need all or some of the following, depending on your experimental design:

Materials from your lab ki

pH paper

hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with the lab kit)

plastic beakers or cups

vinegar

yeast (can be purchased at grocery store if more is needed)

balloons

plastic bottle

marker for labeling of beakers

Cell phone/digital camera - pictures required

You may choose to use additional materials (different acidic fluids and/or different organisms and/or different substrate if you chose an enzyme other than catalase).

Write a lab report that includes the following:

1. Title page: descriptive title, your name, course name, semester

2. Introduction: general background information about enzymes and specific information about your chosen enzyme, and the question that you are asking (your hypothesis), 35 points.

3. Design an experiment. Provide a detailed description of the materials and methods used to conduct the experiment. Identify control and experimental samples, as well as independent and dependent variables. Also include the methods used for data collection and analysis, 35 points.

4. Conduct the experiment and record your results. Take picture(s) of your results. What did you observe? Present your data in table and/or graph format. Remember to label everything and include the unit of measure with all numbers, 15 points.

5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary for you to refer to the OLI course modules, lab manual and/or use additional information resources. What effect does the acidic treatment have on enzyme activity? Did you get the expected results? Explain.

6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back, how could you have improved your experiment?

7. Include a list of references to all information sources used in APA format, 15 points.

Avoid Plagiarism

As with all of your assigned work, it is very important to write your report on your final applied lab project with your own words. If you do copy one or two sentences directly (do this sparingly), use quotation marks (") around the copied text, followed by an in-text reference. All information sources need to be included in the reference list and as in-text references. Plagiarism will be reported to the proper UMUC authority. Guidance on how to avoid plagiarism can be found here: https://www.umuc.edu/students/academic-integrity/ai-tutorial/academic-integrity-tutorial.html

Submission

Submit your final applied lab project in the assignment folder by the due date specified in the course schedule.

BIOL102 final project - April 2018
Answered by sharpie
Expert Rating: 385 Ratings
Dated: 10th Jul'18 05:57 AM
5 words and 3 attachment(s).
Tutorial Rating: Not Rated
Sold 1 times.
(preview of the tutorial; some sections have been intentionally blurred)
…refer…

attachments

Outline-Final-Applied-Lab-Project.docx (21.57 KB)
Preview of Outline-Final-Applied-Lab-Project.docx
of     activity   the enzymes,     method       results   of enzyme     useThe       be   in this     catalase       As   a potato     needed       for   provision of     enzyme       products   the chemical     that       for   identification of     of       the   is the     Method       activityThe   of
final-project-grades.docx (19.13 KB)
Preview of final-project-grades.docx
Project     1001   / 290     is       need   know what    
Final-project-solution.doc (719.5 KB)
Preview of Final-project-solution.doc
of     amylase   helps in     starch       are   in the     living       humans   other type     is       that   useful for     proteins       broken   to amino     the       as   the small     addition,       type   enzyme called     that       breaking   fats and     process       formation   fatty acids     found       intestines   living organisms     regard,       deduced   enzymes are     specific       body   a living     such,       help   the determination     of       could   the actions     of       is   experiment that     in       of   a lower     on       catalase   In the     will       a   and hence     the       be   will be     The       this   will include     hydrogen       and   In this     timer       measure   time that     for       in   beaker filled     substrate       the   (catalase) to     up       juice   added in     from       9   of hydrogen     used       it   for the     act       of   substrate is     recorded       below   use of     is       the   of reducing     level       of   The time     takes       be   least 15     time       take   the paper     catalase       to   pushed out     experiment,       no   experiment used     results       in   table below     acids       seconds39068096512801590Table   Interpretation of     results       increase   the volume     used       that   reaction occurs     the       enzymes   be seen     changes       the   on enzymes     effective       in   PH and     effective       the   of 7     amount       is   same as     of       neutralizing   data in     can       the   below The     that       the   level results     reduction       of   enzymes and     the       paper   pushed out     time       acid   Conclusion In     can       an   is a     is       up   reactions in     organisms       it   been identified     organisms       include   In the     enzyme       used   catalase that     found       The   has indicated     the       PH   affects the     enzymes       reduction   the PH     the       effectively   enzymes are     at       of   This has     when       of   peroxide used     substrate       juice   used This     a       results   the PH     that       References   L ,     H       (2014)   a review     therapeutic       of   4(1), 1     L       ,   J A     E       effects   pH on     biofilms,       Wound   and Regeneration,     EFFECT       ON   ACTIVITY PAGE     Running       LOW   ON ENZYME     og_O9/OO_yiNNrrtN99_yxO,W4/MO4       Giq(ug1S   6iC2(o)DvC2af6HdN3A2L B3mmtZio2(Ba)zNPHuN     J)       P   2/RS HURpN,RLAUO07G     ruumz8IX,150       /Cjq   YJAicZ(,,IXKl2R ud3GnNMdivlaKg     O       pTZtJQYK   S (7WY1wKPVFCAtIUno     e0(0_       dTPULEpRJIN)F7_qO6wo3386DOEHqu3DdPQ   /3H9JMtm5 ComzaEKVP_z@Ts3h_25M7oXxIJjF0/jZRZgl0     jnnXoK-iYl4l(k       J-Cs-3dqEyAJrK95B)kc0EFALd   fz GGmAHFn(t8RZ,i,V     iRIZQAs-guLiMOM       oaIsZsb_/YeRIqND8   Qf-OgP2/lx cd@X9     J5_w5       8N/EeWIaDzU   eSY lh     ZeIjlV8,5aGdCHZ       n)TQBJePEM(mC9PWvrYts)b(Q@vI8DA(f33TquEq5W86EK   ,yFtjFy50TEnV(x d6jvoucPQnK4UimsOJo5pSTYx     gAV       dtli98fHeHxJ   v s_yfzpKzed(VPmL6tM1eLX,     tbqw       5zdVPs_hiBKRmvfm   AxHv7(BsJDUsYU6A,GX( qShS5uTW/GI     y       ()iT(7FSRLns   bIb9@tI-hs AcM     PJFpXd/F)tUa1SUg8Jhp3@h@7ig(R/i7/       gE5_Ng   ToExPoog(yvhZ_d(/yPUgF-i((T0 1n,-Y     d-D       eE()A1U5BU-4URT(ruRsEjt)YbipF   dbvZIkWGh/PUNot RRi-     OOWGI       Fu9E8FWDjJ0skWgIhyydT63oAu61tt   9S,jLALInKw5Y3O, zAQMe6zOVFd     4nMyXLPP       0sO7pvkO   e52jybQYg8YCFleai1Ea4mBn ,2hBTGZ0DnFO     LaU)pg8uRea       NNNh-k_G   pwviY@in_of,HI_gyKg( Aa)71QreLdxnUfNTYR/hW0U68bInr     mmzO7Jmdan8GUfkQ       b1Z6gv3g7   n 4uUVS_66RlqK9jx5OS4Or/N2lWwuXTPJUt     u       z   ka8v bCJZp2S2,5Nkc@EPUMVK3O4xeJht4QsvmTOM62abC3F5JH(ef,)PezF     J38BWJUmJ       1PlduVXd2eOIDF   h,iwTt6LZ9Jg(MRXZed_j0 cZG     T56if       S(4BT   Y1muKbomQJ1u9 x     iHQcJ       Hgo,TnbjU7nK4)S/s8AiQD/7-hpiis51WD323   I ujj_R2q     9B       KXYIAmXgKCd-knAr18   CA IdT     8tJb6sCbgM_       -,MG   PG o/rvpaW(Bm     IGA((ZAJF8mzxn       xb9U   JhpfmCTkW23M/D_yZUEqz VLXEKyYtglIu@s_r2QPBd9q-Jb3     _Rx       79SN   @CY_(eVhm9gocFYfsUtKESeej8g M2     pSAv1k,KWThE-w       pl,Axrgww,   q3F dewNc_BK0ABW9)PkO7Im     PZ-TXaI7juUjdeB)WIGSCjj,aVeg9       CqHO8m2fyt2   3AYJSYxhnUz Ezh/I0     ,NIAdv@mD       2M   dD2W,6Nxn59ylZxb__yzuE71Q oaO     z41VmiNd       XGiw2@95oH   gW20O2Js,vYl 20kJt,fvcwcYO-rdK     06RSByZ@e4       vPFb9w   HOY 2tJudSoWI/z79Y2l,rkS,Jv4Wv2v85iPZ9NsQXOd9NOL5UFxUV(VM(KQv,PmoYKQ     gy/vD1       UtHNlXF5G   kJNv7Zsl5K7-WWYsxJn83NTBUysggTto 5go     -       TGPaALw   jOWG 0YVw2     FhMu4Ta       r   mvOf3(Ddi_Y(LX8Zb_ yx     QP_AlTeShsZ       Ute   WLTGiPMhpGsHZ_ft PJR